Mitokyne : a ratiometric raman probe for mitochondrial pH
Wilson, Liam T. and Tipping, William J. and Wetherill, Corinna and Henley, Zoë and Faulds, Karen and Graham, Duncan and Mackay, Simon P. and Tomkinson, Nicholas C. O. (2021) Mitokyne : a ratiometric raman probe for mitochondrial pH. Analytical Chemistry, 93 (37). pp. 12786-12792. ISSN 0003-2700 (https://doi.org/10.1021/acs.analchem.1c03075)
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Abstract
Mitochondrial pH (pHmito) is intimately related to mitochondrial function and aberrant values for pHmito are linked to several disease states. We report the design, synthesis and application of mitokyne 1 – the first small molecule pHmito sensor for Stimulated Raman Scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pHmito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.
ORCID iDs
Wilson, Liam T., Tipping, William J., Wetherill, Corinna ORCID: https://orcid.org/0000-0001-7263-1352, Henley, Zoë, Faulds, Karen ORCID: https://orcid.org/0000-0002-5567-7399, Graham, Duncan ORCID: https://orcid.org/0000-0002-6079-2105, Mackay, Simon P. ORCID: https://orcid.org/0000-0001-8000-6557 and Tomkinson, Nicholas C. O. ORCID: https://orcid.org/0000-0002-5509-0133;-
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Item type: Article ID code: 77644 Dates: DateEvent21 September 2021Published10 September 2021Published Online26 August 2021AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry
Strategic Research Themes > Health and Wellbeing
Technology and Innovation Centre > Bionanotechnology
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical SciencesDepositing user: Pure Administrator Date deposited: 02 Sep 2021 15:32 Last modified: 11 Nov 2024 13:12 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/77644