Mitokyne : a ratiometric raman probe for mitochondrial pH

Wilson, Liam T. and Tipping, William J. and Wetherill, Corinna and Henley, Zoë and Faulds, Karen and Graham, Duncan and Mackay, Simon P. and Tomkinson, Nicholas C. O. (2021) Mitokyne : a ratiometric raman probe for mitochondrial pH. Analytical Chemistry, 93 (37). pp. 12786-12792. ISSN 0003-2700 (https://doi.org/10.1021/acs.analchem.1c03075)

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Abstract

Mitochondrial pH (pHmito) is intimately related to mitochondrial function and aberrant values for pHmito are linked to several disease states. We report the design, synthesis and application of mitokyne 1 – the first small molecule pHmito sensor for Stimulated Raman Scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pHmito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.

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