Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance

Kim, Suhee and Wark, Alastair W. and Lee, Hye Jin (2016) Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance. Analytical Chemistry, 88 (15). pp. 7793-7799. ISSN 0003-2700 (https://doi.org/10.1021/acs.analchem.6b01825)

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Abstract

The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer’s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.

  • Item type:Article
    ID code:58346
    Dates:
    Date
    Event
    11 July 2016
    Published
    11 July 2016
    Accepted
    Notes:This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Analytical Chemistry, copyright © American Chemical Society after peer review. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01825
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:28 Oct 2016 11:08
    Last modified:18 Apr 2024 00:19
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/58346
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