Ultrasensitive DNA detection using oligonucleotide-silver nanoparticle conjugates

Thompson, D.G. and Enright, A. and Faulds, K. and Smith, W.E. and Graham, D. (2008) Ultrasensitive DNA detection using oligonucleotide-silver nanoparticle conjugates. Analytical Chemistry, 80 (8). pp. 2805-2810. ISSN 0003-2700 (https://doi.org/10.1021/ac702403w)

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Abstract

Oligonucleotide−gold nanoparticle (OGN) conjugates are powerful tools for the detection of target DNA sequences due to the unique properties conferred upon the oligonucleotide by the nanoparticle. Practically all the research and applications of these conjugates have used gold nanoparticles to the exclusion of other noble metal nanoparticles. Here we report the synthesis of oligonucleotide−silver nanoparticle (OSN) conjugates and demonstrate their use in a sandwich assay format. The OSN conjugates have practically identical properties to their gold analogues and due to their vastly greater extinction coefficient both visual and absorption analyses can occur at much lower concentrations. This is the first report of OSN conjugates being successfully used for target DNA detection and offers improved sensitivity which is of interest to a range of scientists.

ORCID iDs

Thompson, D.G., Enright, A., Faulds, K. ORCID logoORCID: https://orcid.org/0000-0002-5567-7399, Smith, W.E. and Graham, D. ORCID logoORCID: https://orcid.org/0000-0002-6079-2105;
  • Item type:Article
    ID code:17082
    Dates:
    Date
    Event
    15 April 2008
    Published
    Notes:http://suprimo.lib.strath.ac.uk/primo_library/libweb/action/display.do?ct=display&doc=SUSFX954925379953&indx=12&vl(freeText0)=Analytical%20Chemistry&fn=search&fctV=include%20only%20journals%20or%20serials&tab=local&fctN=facet_fmt&scp.scps=scope%3A(SU)&mode=Basic&dscnt=0&ct=Next%20Page&vl(54032236UI0)=lsr02&vl(96071691UI1)=all_items&dstmp=1272467296691&frbg=&srt=popularity&indx=11&dum=true&vid=SUVU01
    Subjects:Science > Chemistry
    Science > Chemistry > Physical and theoretical chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Strathprints Administrator
    Date deposited:28 Apr 2010 15:22
    Last modified:11 Nov 2024 09:21
    URI:https://strathprints.strath.ac.uk/id/eprint/17082
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