Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups

Mackellar, C. and Graham, D. and Will, D.W. and Burgess, S. and Brown, T. (1992) Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups. Nucleic Acids Research, 20 (13). pp. 3411-3417. ISSN 1362-4962 (http://dx.doi.org/10.1093/nar/20.13.3411)

Full text not available in this repository.Request a copy

Abstract

A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.

ORCID iDs

Mackellar, C., Graham, D. ORCID logoORCID: https://orcid.org/0000-0002-6079-2105, Will, D.W., Burgess, S. and Brown, T.;
CORE (COnnecting REpositories)