Application of atypical acetyl-lysine methyl mimetics in the development of selective inhibitors of the bromodomain-containing protein 7 (BRD7)/bromodomain-containing protein 9 (BRD9) bromodomains

Clegg, Michael A. and Bamborough, Paul and Chung, Chun-wa and Craggs, Peter D. and Gordon, Laurue and Grandi, Paola and Leveridge, Melanie and Lindon, Matthew and Liwicki, Gemma M. and Michon, Anne-Marie and Molnar, Judit and Rioja, Inmaculada and Soden, Peter E. and Theodoulou, Natalie H. and Werner, Thilo and Tomkinson, Nicholas C. O. and Prinjha, Rab K. and Humphreys, Philip G. (2020) Application of atypical acetyl-lysine methyl mimetics in the development of selective inhibitors of the bromodomain-containing protein 7 (BRD7)/bromodomain-containing protein 9 (BRD9) bromodomains. Journal of Medicinal Chemistry, 63 (11). pp. 5816-5840. ISSN 0022-2623 (https://doi.org/10.1021/acs.jmedchem.0c00075)

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Abstract

Non-BET bromodomain containing proteins have become attractive targets for the development of novel therapeutics targeting epigenetic pathways. To help facilitate the target validation of this class of proteins, structurally diverse small molecule ligands, and methodologies to produce selective inhibitors in a predictable fashion are in high demand. Herein we report the development and application of atypical acetyl-lysine (KAc) methyl mimetics to take advantage of the differential stability of conserved water molecules in the bromodomain binding side. Discovery of the n-butyl group as an atypical KAc methyl mimetic allowed generation of 31 (GSK6776) as a soluble, permeable and selective BRD7/9 inhibitor from a pyridazinone template. The n-butyl group was then used to enhance the bromodomain selectivity of an existing BRD9 inhibitor and to transform pan-bromodomain inhibitors into BRD7/9 selective compounds. Finally, a solvent exposed vector was defined from the pyridazinone template to enable bifunctional molecule synthesis and affinity enrichment chemoproteomic experiments were used to confirm several of the endogenous protein partners of BRD7 and BRD9 which form part of the chromatin remodelling PBAF and BAF complexes, respectively.

ORCID iDs

Clegg, Michael A., Bamborough, Paul, Chung, Chun-wa, Craggs, Peter D., Gordon, Laurue, Grandi, Paola, Leveridge, Melanie, Lindon, Matthew, Liwicki, Gemma M., Michon, Anne-Marie, Molnar, Judit, Rioja, Inmaculada, Soden, Peter E., Theodoulou, Natalie H., Werner, Thilo, Tomkinson, Nicholas C. O. ORCID logoORCID: https://orcid.org/0000-0002-5509-0133, Prinjha, Rab K. and Humphreys, Philip G.;
  • Item type:Article
    ID code:73247
    Dates:
    Date
    Event
    11 June 2020
    Published
    15 May 2020
    Published Online
    23 April 2020
    Accepted
    Notes:This document is the Accepted Manuscript version of a Published Work that appeared in final form in the Journal of Medicinal Chemistry, copyright © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work visit: https://doi.org/10.1021/acs.jmedchem.0c00075
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:16 Jul 2020 13:29
    Last modified:11 Nov 2024 12:46
    URI:https://strathprints.strath.ac.uk/id/eprint/73247
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