Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance

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Kim, Suhee and Wark, Alastair W. and Lee, Hye Jin (2016) Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance. Analytical Chemistry, 88 (15). pp. 7793-7799. ISSN 0003-2700 (https://doi.org/10.1021/acs.analchem.6b01825)

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Abstract

The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer’s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.

ORCID iDs

Kim, Suhee, Wark, Alastair W. ORCID logoORCID: https://orcid.org/0000-0001-8736-7566 and Lee, Hye Jin;
  • Item type:Article
    ID code:58346
    Dates:
    Date
    Event
    11 July 2016
    Published
    11 July 2016
    Accepted
    Notes:This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Analytical Chemistry, copyright © American Chemical Society after peer review. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01825
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:28 Oct 2016 11:08
    Last modified:12 Dec 2024 04:49
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/58346
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