Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth : the role of sphingosine 1-phosphate receptor-4

Ohotski, Jan and Rosen, Hugh and Bittman, Robert and Pyne, Susan and Pyne, Nigel J (2014) Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth : the role of sphingosine 1-phosphate receptor-4. Cellular Signalling, 26 (5). pp. 1040-1047. ISSN 1873-3913 (https://doi.org/10.1016/j.cellsig.2014.01.023)

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Abstract

We demonstrate that pre-treatment of estrogen receptor negative MDA-MB-231 breast cancer cells containing ectopically expressed HA-tagged sphingosine 1-phosphate receptor-2 (S1P2) with the sphingosine kinase 1/2 inhibitor SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) or the sphingosine kinase 2 selective inhibitor (R)-FTY720 methyl ether (ROMe) or sphingosine kinase 2 siRNA induced the translocation of HA-tagged S1P2 and Y416 phosphorylated c-Src to the nucleus of these cells. This is associated with reduced growth of HA-tagged S1P2 over-expressing MDA-MB-231 cells. Treatment of HA-S1P2 over-expressing MDA-MB-231 cells with the sphingosine 1-phosphate receptor-4 (S1P4) antagonist CYM50367 or with S1P4 siRNA also promoted nuclear translocation of HA-tagged S1P2. These findings identify for the first time a signaling pathway in which sphingosine 1-phosphate formed by sphingosine kinase 2 binds to S1P4 to prevent nuclear translocation of S1P2 and thereby promote the growth of estrogen receptor negative breast cancer cells.

ORCID iDs

Ohotski, Jan, Rosen, Hugh, Bittman, Robert, Pyne, Susan ORCID logoORCID: https://orcid.org/0000-0002-6608-9584 and Pyne, Nigel J ORCID logoORCID: https://orcid.org/0000-0002-5657-4578;
  • Item type:Article
    ID code:49212
    Dates:
    Date
    Event
    May 2014
    Published
    29 January 2014
    Published Online
    24 January 2014
    Accepted
    Notes:Notice: This is the author's version of a work that was accepted for publication in Cellular Signalling. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cellular Signalling, Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth: the role of sphingosine 1-phosphate receptor-4 Ohotski, J., Rosen, H., Bittman, R., Pyne, S. & Pyne, N. J. May 2014. 26, 5, p. 1040-1047, DOI: 10.1016/j.cellsig.2014.01.023.
    Subjects:Medicine > Pharmacy and materia medica
    Medicine > Therapeutics. Pharmacology
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:15 Sep 2014 09:02
    Last modified:11 Nov 2024 10:46
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/49212
CORE (COnnecting REpositories)