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Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides

Macaskill, A. and Chernonosov, A.A and Koval, V.V and Lukyanets, E.A. and Fedorova, O.S. and Smith, W.E. and Faulds, K. and Graham, D. (2007) Quantitative surface-enhanced resonance raman scattering of phthalocyanine labelled oligonucleotides. Nucleic Acids Research, 35 (6). E42. ISSN 0305-1048

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The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10-11 mol. dm-3 were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.