High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics

Davidson, Callum G. and Punnabhum, Panida and Farrell, Gillian and Perrie, Yvonne and Rattray, Zahra (2024) High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics. In: JPAG Pharmaceutical Analysis Research Awards and Careers Fair 2024, 2024-11-12 - 2024-11-12.

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Abstract

Aim: The aim of this study is to develop an orthogonal analytical pipeline for the analysis of manufacture associated impurities and RNA API critical quality attributes using Nusinersen. Objectives: • Develop and optimise hydrophilic interaction liquid chromatography. • Develop orthogonal analytical method pipeline to evaluate antisense oligonucleotide beyond the scope of routine techniques. • Compare results between orthogonal high resolution analytical techniques.

ORCID iDs

Davidson, Callum G. ORCID logoORCID: https://orcid.org/0009-0003-8736-9918, Punnabhum, Panida ORCID logoORCID: https://orcid.org/0009-0002-6830-1072, Farrell, Gillian, Perrie, Yvonne and Rattray, Zahra ORCID logoORCID: https://orcid.org/0000-0002-8371-8549;
CORE (COnnecting REpositories)