Piperacillin/tazobactam-resistant, cephalosporin-susceptible Escherichia coli bloodstream infections are driven by multiple acquisition of resistance across diverse sequence types

Edwards, Thomas and Heinz, Eva and van Aartsen, Jon and Howard, Alex and Roberts, Paul and Corless, Caroline and Fraser, Alice J. and Williams, Christopher T. and Bulgasim, Issra and Cuevas, Luis E. and Parry, Christopher M. and Roberts, Adam P. and Adams, Emily R. and Mason, Jenifer and Hubbard, Alasdair T.M. (2022) Piperacillin/tazobactam-resistant, cephalosporin-susceptible Escherichia coli bloodstream infections are driven by multiple acquisition of resistance across diverse sequence types. Microbial Genomics, 8 (4). 000789. ISSN 2057-5858 (https://doi.org/10.1099/mgen.0.000789)

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Abstract

Resistance to piperacillin/tazobactam (TZP) in Escherichia coli has predominantly been associated with mechanisms that confer resistance to third-generation cephalosporins. Recent reports have identified E. coli strains with phenotypic resistance to piperacillin/tazobactam but susceptibility to third-generation cephalosporins (TZP-R/3GC-S). In this study we sought to determine the genetic diversity of this phenotype in E. coli (n=58) isolated between 2014–2017 at a single tertiary hospital in Liverpool, UK, as well as the associated resistance mechanisms. We compare our findings to a UK-wide collection of invasive E. coli isolates (n=1509) with publicly available phenotypic and genotypic data. These data sets included the TZP-R/3GC-S phenotype (n=68), and piperacillin/tazobactam and third-generation cephalosporin-susceptible (TZP-S/3GC-S, n=1271) phenotypes. The TZP-R/3GC-S phenotype was displayed in a broad range of sequence types, which was mirrored in the same phenotype from the UK-wide collection, and the overall diversity of invasive E. coli isolates. The TZP-R/3GC-S isolates contained a diverse range of plasmids, indicating multiple acquisition events of TZP resistance mechanisms rather than clonal expansion of a particular plasmid or sequence type. The putative resistance mechanisms were equally diverse, including hyperproduction of TEM-1, either via strong promoters or gene amplification, carriage of inhibitor-resistant β-lactamases, and an S133G blaCTX-M-15 mutation detected for the first time in clinical isolates. Several of these mechanisms were present at a lower abundance in the TZP-S/3GC-S isolates from the UK-wide collection, but without the associated phenotypic resistance to TZP. Eleven (19%) of the isolates had no putative mechanism identified from the genomic data. Our findings highlight the complexity of this cryptic phenotype and the need for continued phenotypic monitoring, as well as further investigation to improve detection and predic-tion of the TZP-R/3GC-S phenotype from genomic data.

ORCID iDs

Edwards, Thomas, Heinz, Eva ORCID logoORCID: https://orcid.org/0000-0003-4413-3756, van Aartsen, Jon, Howard, Alex, Roberts, Paul, Corless, Caroline, Fraser, Alice J., Williams, Christopher T., Bulgasim, Issra, Cuevas, Luis E., Parry, Christopher M., Roberts, Adam P., Adams, Emily R., Mason, Jenifer and Hubbard, Alasdair T.M.;
  • Item type:Article
    ID code:90652
    Dates:
    Date
    Event
    11 April 2022
    Published
    1 February 2022
    Accepted
    Subjects:Science > Microbiology
    Department:UNSPECIFIED
    Depositing user: Pure Administrator
    Date deposited:23 Sep 2024 13:16
    Last modified:11 Nov 2024 14:27
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/90652
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