Key environmental exposure pathways to antimicrobial resistant bacteria in southern Malawi : a SaniPath approach
Mwapasa, Taonga and Chidziwisano, Kondwani Regson and Mphasa, Madalitso and Cocker, Derek and Rimella, Lorenzo and Feasey, Nicholas and Morse, Tracy (2024) Key environmental exposure pathways to antimicrobial resistant bacteria in southern Malawi : a SaniPath approach. Science of the Total Environment, 945. 174142. ISSN 1879-1026 (https://doi.org/10.1016/j.scitotenv.2024.174142)
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Abstract
Antimicrobial resistance (AMR) poses a severe global health threat, yet the transmission pathways of AMR within communal public environments, where humans and animals interact, remain poorly explored. This study investigated AMR risk pathways, prevalence, and seasonality of extended-spectrum β-lactamase (ESBL) producing E. coli and K. pneumoniae, and observed practices contributing to environmental contamination within urban, peri-urban, and rural Malawi. Using the SaniPath tool, in August 2020, transect walks across three Malawian study sites identified potential AMR exposure pathways, including drains, standing water, soil, and areas of communal hand contact. Subsequently, from September-2020 to August-2021, 1440 environmental samples were collected at critical points along exposure routes (n = 40/month from each site). These underwent microbiological analysis using chromogenic agar techniques to detect the presence of ESBL E. coli and ESBL K. pneumoniae. Results showed the highest ESBL prevalence in urban environments (68.1 %, 95%CI = 0.64–0.72, p < 0.001) with a higher ESBL presence seen in drains (58.8 %, 95%CI = 055–0.62, p < 0.001) and soil (54.1 %, 95%CI = 0.46–0.62, p < 0.001) compared to other pathways. Environmental contamination was attributed to unavailability and poor condition of sanitation and hygiene infrastructure based on key informant interviews with community leaders (n = 9) and confirmed by independent observation. ESBL prevalence varied between seasons (χ 2 (2,N = 1440) = 10.89, p = 0.004), with the highest in the hot-dry period (55.8 % (n = 201)). Prevalence also increased with increased rainfall (for ESBL E.coli). We highlight that community environments are likely to be a crucial component in AMR transmission, evident in the abundance of ESBL bacteria in identified exposure pathways. Additionally, poor sanitation infrastructure and practices coupled with seasonal dynamics further affect the presence of ESBLs in communal environments. Therefore, a context appropriate whole system approach that tackles infrastructure and behavioural factors, supported by effective surveillance is required to impact AMR and a range of aligned development challenges in these settings.
ORCID iDs
Mwapasa, Taonga, Chidziwisano, Kondwani Regson, Mphasa, Madalitso, Cocker, Derek, Rimella, Lorenzo, Feasey, Nicholas and Morse, Tracy ORCID: https://orcid.org/0000-0003-4185-9471;-
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Item type: Article ID code: 89706 Dates: DateEvent1 October 2024Published21 June 2024Published Online17 June 2024Accepted15 March 2024SubmittedSubjects: Geography. Anthropology. Recreation > Environmental Sciences
Science > Microbiology
Geography. Anthropology. Recreation > Geography (General)Department: Faculty of Engineering > Civil and Environmental Engineering Depositing user: Pure Administrator Date deposited: 24 Jun 2024 08:46 Last modified: 14 Dec 2024 13:34 URI: https://strathprints.strath.ac.uk/id/eprint/89706