Protective effects of madecassoside, a triterpenoid from Centella asiatica, against oxidative stress in INS-1E cells

Tan, Swee Ching and Rajendran, Ramkumar and Bhattamisra, Subrat Kumar and Krishnappa, Purushotham and Davamani, Fabian and Chitra, Ebenezer and Ambu, Stephen and Furman, Brian and Candasamy, Mayuren (2024) Protective effects of madecassoside, a triterpenoid from Centella asiatica, against oxidative stress in INS-1E cells. Natural Product Research. ISSN 1478-6427 (https://doi.org/10.1080/14786419.2024.2315499)

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Abstract

Progressive decline in β cell function and reduction in the β cell mass is important in type 2 diabetes. Here, we tested the hypothesis that madecassoside’s previously demonstrated in vivo protective effects on the β cell in experimental diabetes were exerted directly. We investigated the effects of madecassoside in protecting a β cell line (INS-1E) against a variety of agents. INS-1E cells were treated with madecassoside in the presence of high glucose (HG), a cytokine mixture, hydrogen peroxide (H2O2), or streptozotocin (STZ). HG, the cytokine mixture, H2O2 and STZ each produced a significant decrease in cell viability; this was significantly reversed by madecassoside. Pre-treatment with madecassoside reduced the number of apoptotic cells induced by HG, the cytokine mixture, H2O2, and STZ, and concentration-dependently reduced ROS production. Madecassoside also significantly enhanced glucose-induced insulin secretion. The results suggest that madecassoside’s in vivo effects are exerted directly on the β cell.

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