Development of a high-accuracy, low-cost, and portable fluorometer with smartphone application for the detection of urinary albumin towards the early screening of chronic kidney and renal diseases

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Pinrod, Visarute and Chawjiraphan, Wireeya and Segkhoonthod, Khoonsake and Hanchaisri, Kriangkai and Tantiwathanapong, Phornpol and Pinpradup, Preedee and Putnin, Thitirat and Pimalai, Dechnarong and Treerattrakoon, Kiatnida and Cha’on, Ubon and Anutrakulchai, Sirirat and Japrung, Deanpen (2023) Development of a high-accuracy, low-cost, and portable fluorometer with smartphone application for the detection of urinary albumin towards the early screening of chronic kidney and renal diseases. Biosensors, 13 (9). 876. ISSN 2079-6374 (https://doi.org/10.3390/bios13090876)

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Abstract

This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor, the device achieved a linear calibration curve (0.001–1.5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 µg/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study’s fluorometer, a commercial fluorometer, and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover, the fluorometer’s portability offers potential applications in portable point-of-care testing, enhancing its utility in clinical settings for early disease screening.

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