Selective cellular imaging with lanthanide‐based upconversion nanoparticles

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Nampi, Padmaja P. and Vakurov, Alexander and MacKenzie, Lewis E. and Scrutton, Nigel S. and Millner, Paul A. and Jose, Gin and Saha, Sikha (2018) Selective cellular imaging with lanthanide‐based upconversion nanoparticles. Journal of Biophotonics, 12 (4). pp. 1-9. e201800256. ISSN 1864-0648 (https://doi.org/10.1002/jbio.201800256)

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Abstract

Upconversion nanoparticles (UCNPs) with sodium yttrium fluoride, NaYF4 (host lattice) doped with Yb3+ (sensitizer) and Er3+ (activator) were synthesized via hydrothermal route incorporating polyethyleneimine (PEI) for their long-term stability in water. The cationic PEI-modified UCNPs with diameter 20 ± 4 nm showed a zeta potential value of +36.5 mV and showed an intense, visible red luminescence and low-intensity green emission with 976 nm laser excitation. The particles proven to be nontoxic to endothelial cells, with a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, showing 90% to 100% cell viability, across a wide range of UCNP concentrations (0.3 ng/mL-0.3 mg/mL) were used in multiphoton imaging. Multiphoton cellular imaging and emission spectroscopy data reported here prove that the UCNPs dispersed in cell culture media are predominantly concentrated in the cytoplasm than the cell nucleus. The energy transfer from PEI-coated UCNPs to surrounding media for red luminescence in the biological system is also highlighted with spectroscopic measurements. Results of this study propose that UCNPs can, therefore, be used for cytoplasm selective imaging together with multiphoton dyes (eg, 4′,6-diamidino-2-phenylindole (DAPI)) that are selective to cell nucleus.

ORCID iDs

Nampi, Padmaja P., Vakurov, Alexander, MacKenzie, Lewis E. ORCID logoORCID: https://orcid.org/0000-0002-8151-0525, Scrutton, Nigel S., Millner, Paul A., Jose, Gin and Saha, Sikha;
  • Item type:Article
    ID code:77953
    Dates:
    Date
    Event
    23 October 2018
    Published
    20 October 2018
    Accepted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:30 Sep 2021 12:14
    Last modified:11 Nov 2024 13:14
    URI:https://strathprints.strath.ac.uk/id/eprint/77953
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