A photoaffinity displacement assay and probes to study the cyclin‐dependent kinase family

Grant, Emma K. and Fallon, David J. and Eberl, H. Christian and Fantom, Ken G. M. and Zappacosta, Francesca and Messenger, Cassie and Tomkinson, Nicholas C. O. and Bush, Jacob T. (2019) A photoaffinity displacement assay and probes to study the cyclin‐dependent kinase family. Angewandte Chemie International Edition, 58 (48). pp. 17322-17327. ISSN 1521-3773 (https://doi.org/10.1002/anie.201906321)

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Abstract

The CDK family plays a crucial role in the control of the cell cycle. Dysregulation and mutation of the CDKs has been implicated in cancer and the CDKs have been investigated extensively as potential therapeutic targets. Selective inhibition of specific isoforms of the CDKs is crucial to achieve therapeutic effect while minimising toxicity. We present a group of photoaffinity probes designed to bind to the family of CDKs. The site of crosslinking of the optimised probe, as well as its ability to enrich members of the CDK family from cell lysates, was investigated. In a proof of concept study, we subsequently developed a photoaffinity probe‐based competition assay to profile CDK inhibitors. We anticipate that this approach will be widely applicable to the study of small molecule binding to protein families of interest.

ORCID iDs

Grant, Emma K., Fallon, David J., Eberl, H. Christian, Fantom, Ken G. M., Zappacosta, Francesca, Messenger, Cassie, Tomkinson, Nicholas C. O. ORCID logoORCID: https://orcid.org/0000-0002-5509-0133 and Bush, Jacob T.;
  • Item type:Article
    ID code:71896
    Dates:
    Date
    Event
    25 November 2019
    Published
    13 October 2019
    Published Online
    13 September 2019
    Accepted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:26 Mar 2020 11:51
    Last modified:11 Nov 2024 12:29
    URI:https://strathprints.strath.ac.uk/id/eprint/71896
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