Stability-indicating micellar enhanced spectro-fluorometric determination of Daclatasvir in its tablet and spiked human plasma
Sultan, Maha A. and El-Alamin, Maha M. Abou and Wark, Alastair W. and Azab, Marwa M. (2019) Stability-indicating micellar enhanced spectro-fluorometric determination of Daclatasvir in its tablet and spiked human plasma. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 211. pp. 52-58. ISSN 1386-1425 (https://doi.org/10.1016/j.saa.2018.11.023)
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Abstract
A fast, simple and sensitive micellar enhanced spectrofluorimetric method is performed for the determination of Daclatasvir dihydrochloride (DAC) in its pharmaceutical dosage form and in spiked human plasma. The fluorescence intensity (FI) was measured at 367 nm after excitation at 300 nm. In aqueous solution, the FI of DAC was greatly enhanced by >110% in the presence of sodium dodecyl sulphate (SDS). The detection method was linear over the range of 12.93 to 161.60 ng/mL, with a limit of detection of 1.75 ng/mL. The proposed method was successfully applied to the determination of DAC in its pharmaceutical dosage form and the mean % recovery of DAC in spiked human plasma was 95.42 ± 2.52. The developed methodology was also extended to stress studies of DAC after exposure to different forced degradation conditions including acidic, alkaline, photolytic, thermal and oxidative environments.
ORCID iDs
Sultan, Maha A., El-Alamin, Maha M. Abou, Wark, Alastair W. ORCID: https://orcid.org/0000-0001-8736-7566 and Azab, Marwa M.;-
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Item type: Article ID code: 66599 Dates: DateEvent15 March 2019Published17 November 2018Published Online12 November 2018AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 16 Jan 2019 13:04 Last modified: 27 Sep 2024 01:10 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/66599