Methodological considerations for large-scale breath analysis studies : lessons from the U-BIOPRED severe asthma project

Ahmed, Waqar M and Brinkman, Paul and Weda, Hans and Knobel, Hugo H and Xu, Yun and Nijsen, Tamara M and Goodacre, Roy and Rattray, Nicholas and Vink, Teunis J and Santonico, Marco and Pennazza, Giorgio and Montuschi, Paulo and Sterk, Peter J and Fowler, Stephen J (2018) Methodological considerations for large-scale breath analysis studies : lessons from the U-BIOPRED severe asthma project. Journal of Breath Research, 13 (1). ISSN 1752-7163 (https://doi.org/10.1088/1752-7163/aae557)

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Abstract

Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.

ORCID iDs

Ahmed, Waqar M, Brinkman, Paul, Weda, Hans, Knobel, Hugo H, Xu, Yun, Nijsen, Tamara M, Goodacre, Roy, Rattray, Nicholas ORCID logoORCID: https://orcid.org/0000-0002-3528-6905, Vink, Teunis J, Santonico, Marco, Pennazza, Giorgio, Montuschi, Paulo, Sterk, Peter J and Fowler, Stephen J;
CORE (COnnecting REpositories)