A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

Trägårdh, J. and Schniete, J. and Parsons, M. and McConnell, G. (2016) A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging. APL Photonics, 1 (9). 091303. ISSN 2378-0967 (https://doi.org/10.1063/1.4962207)

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Abstract

We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.

ORCID iDs

Trägårdh, J. ORCID logoORCID: https://orcid.org/0000-0002-8662-9509, Schniete, J., Parsons, M. and McConnell, G. ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
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