Protease-catalyzed peptide synthesis on solid support

Ulijn, R.V. and Baragana, B. and Halling, P.J. and Flitsch, S.L. (2002) Protease-catalyzed peptide synthesis on solid support. Journal of the American Chemical Society, 124 (37). pp. 10988-10989. ISSN 1520-5126 (http://dx.doi.org/10.1021/ja026912d)

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Abstract

The direct enzymatic synthesis of peptides from amino acids is widely used as a useful alternative to chemical synthesis. However, good yields of such enzyme-catalyzed reactions require altered reaction conditions to overcome the bias for hydrolysis in aqueous medium. We argue that the synthesis/hydrolysis equilibrium can be shifted toward synthesis in aqueous medium by immobilizing the amine on solid support. In this report, we show the first examples of solid-phase peptide synthesis catalyzed by a protease in bulk aqueous buffer.

ORCID iDs

Ulijn, R.V. ORCID logoORCID: https://orcid.org/0000-0001-7974-3779, Baragana, B., Halling, P.J. ORCID logoORCID: https://orcid.org/0000-0001-5077-4088 and Flitsch, S.L.;
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