Determining biomolecular structures by time-resolved fluorescence

Rolinski, O.J. and Hernandez-Santana, A. and Graham, D. (2006) Determining biomolecular structures by time-resolved fluorescence. Proceedings of SPIE - The International Society for Optical Engineering, 6047 (60472N). (http://dx.doi.org/10.1117/12.710984)

Abstract

This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

ORCID iDs

Rolinski, O.J. ORCID: https://orcid.org/0000-0002-7838-779X

Graham, D. ORCID: https://orcid.org/0000-0002-6079-2105

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• Item metadata

  Item type:	Article
  ID code:	18173
  Dates:	Date Event 2006 Published
  Notes:	Fourth International Conference on Photonics and Imaging in Biology and Medicine. Subseries of Proceedings of SPIE - International Society for Optical Engineering. Vol. 6047, Part 2. Progress in Biomedical Optics and Imaging, Vol. 7(2) Subseries of Proceedings of SPIE.
  Subjects:	Science > Physics > Optics. Light
  Department:	Faculty of Science > Physics Faculty of Science > Pure and Applied Chemistry
  Depositing user:	Strathprints Administrator
  Date deposited:	14 Apr 2010 14:53
  Last modified:	11 Nov 2024 09:18
  Related URLs:	http://direct.bl.uk/bld/PlaceOrder.do?UI... Organisation
  URI:	https://strathprints.strath.ac.uk/id/eprint/18173