Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

McKenzie, F. and Steven, V. and Ingram, A. and Graham, D. (2009) Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis. Chemical Communications (London) (20). pp. 2872-2874. ISSN 0009-241X (http://dx.doi.org/10.1039/b823057a)

Abstract

A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.

ORCID iDs

Graham, D. ORCID: https://orcid.org/0000-0002-6079-2105

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• Item metadata

  Item type:	Article
  ID code:	16953
  Dates:	Date Event 31 March 2009 Published
  Subjects:	Science > Chemistry
  Department:	Faculty of Science > Pure and Applied Chemistry
  Depositing user:	Strathprints Administrator
  Date deposited:	02 Jun 2010 10:34
  Last modified:	11 Nov 2024 09:25
  URI:	https://strathprints.strath.ac.uk/id/eprint/16953