Structure of the bacterial plant-ferredoxin receptor FusA
Grinter, Rhys and Josts, Inokentijs and Mosbahi, Khedidja and Roszak, Aleksander W. and Cogdell, Richard J. and Bonvin, Alexandre M.J.J. and Milner, Joel J. and Kelly, Sharon M. and Byron, Olwyn and Smith, Brian O. and Walker, Daniel (2016) Structure of the bacterial plant-ferredoxin receptor FusA. Nature Communications, 7. 13308. ISSN 2041-1723 (https://doi.org/10.1038/ncomms13308)
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Abstract
Iron is a limiting nutrient in bacterial infection putting it at the centre of an evolutionary arms race between host and pathogen. Gram-negative bacteria utilize TonB-dependent outer membrane receptors to obtain iron during infection. These receptors acquire iron either in concert with soluble iron-scavenging siderophores or through direct interaction and extraction from host proteins. Characterization of these receptors provides invaluable insight into pathogenesis. However, only a subset of virulence-related TonB-dependent receptors have been currently described. Here we report the discovery of FusA, a new class of TonB-dependent receptor, which is utilized by phytopathogenic Pectobacterium spp. to obtain iron from plant ferredoxin. Through the crystal structure of FusA we show that binding of ferredoxin occurs through specialized extracellular loops that form extensive interactions with ferredoxin. The function of FusA and the presence of homologues in clinically important pathogens suggests that small iron-containing proteins represent an iron source for bacterial pathogens.
ORCID iDs
Grinter, Rhys, Josts, Inokentijs, Mosbahi, Khedidja, Roszak, Aleksander W., Cogdell, Richard J., Bonvin, Alexandre M.J.J., Milner, Joel J., Kelly, Sharon M., Byron, Olwyn, Smith, Brian O. and Walker, Daniel ORCID: https://orcid.org/0000-0002-4206-2942;-
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Item type: Article ID code: 86405 Dates: DateEvent31 October 2016Published21 September 2016AcceptedSubjects: Science > Microbiology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 08 Aug 2023 08:48 Last modified: 31 Aug 2024 01:15 URI: https://strathprints.strath.ac.uk/id/eprint/86405