A highly conserved bacterial D-serine uptake system links host metabolism and virulence
Connolly, James P. R. and Gabrielsen, Mads and Goldstone, Robert J. and Grinter, Rhys and Wang, Dai and Cogdell, Richard J. and Walker, Daniel and Smith, David G. E. and Roe, Andrew J. (2016) A highly conserved bacterial D-serine uptake system links host metabolism and virulence. PLOS Pathogens, 12 (1). e1005359. ISSN 1553-7366 (https://doi.org/10.1371/journal.ppat.1005359)
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Abstract
The ability of any organism to sense and respond to challenges presented in the environment is critically important for promoting or restricting colonization of specific sites. Recent work has demonstrated that the host metabolite D-serine has the ability to markedly influence the outcome of infection by repressing the type III secretion system of enterohaemorrhagic Escherichia coli (EHEC) in a concentration-dependent manner. However, exactly how EHEC monitors environmental D-serine is not understood. In this work, we have identified two highly conserved members of the E. coli core genome, encoding an inner membrane transporter and a transcriptional regulator, which collectively help to “sense” levels of D-serine by regulating its uptake from the environment and in turn influencing global gene expression. Both proteins are required for full expression of the type III secretion system and diversely regulated prophage-encoded effector proteins demonstrating an important infection-relevant adaptation of the core genome. We propose that this system acts as a key safety net, sampling the environment for this metabolite, thereby promoting colonization of EHEC to favorable sites within the host.
ORCID iDs
Connolly, James P. R., Gabrielsen, Mads, Goldstone, Robert J., Grinter, Rhys, Wang, Dai, Cogdell, Richard J., Walker, Daniel ORCID: https://orcid.org/0000-0002-4206-2942, Smith, David G. E. and Roe, Andrew J.;-
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Item type: Article ID code: 86402 Dates: DateEvent4 January 2016Published3 December 2015AcceptedSubjects: Science > Microbiology > Virology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 07 Aug 2023 13:48 Last modified: 01 Sep 2024 01:42 URI: https://strathprints.strath.ac.uk/id/eprint/86402