Structural and biophysical analysis of nuclease protein antibiotics

Klein, Alexander and Wojdyla, Justyna Aleksandra and Joshi, Amar and Josts, Inokentijs and McCaughey, Laura C. and Housden, Nicholas G. and Kaminska, Renata and Byron, Olwyn and Walker, Daniel and Kleanthous, Colin (2016) Structural and biophysical analysis of nuclease protein antibiotics. Biochemical Journal, 476 (18). pp. 2799-2812. ISSN 0264-6021 (https://doi.org/10.1042/BCJ20160544)

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Abstract

Protein antibiotics (bacteriocins) are a large and diverse family of multidomain toxins that kill specific Gram-negative bacteria during intraspecies competition for resources. Our understanding of the mechanism of import of such potent toxins has increased significantly in recent years, especially with the reporting of several structures of bacteriocin domains. Less well understood is the structural biochemistry of intact bacteriocins and how these compare across bacterial species. Here, we focus on endonuclease (DNase) bacteriocins that target the genomes of Escherichia coli and Pseudomonas aeruginosa, known as E-type colicins and S-type pyocins, respectively, bound to their specific immunity (Im) proteins. First, we report the 3.2 Å structure of the DNase colicin ColE9 in complex with its ultra-high affinity Im protein, Im9. In contrast with Im3, which when bound to the ribonuclease domain of the homologous colicin ColE3 makes contact with the translocation (T) domain of the toxin, we find that Im9 makes no such contact and only interactions with the ColE9 cytotoxic domain are observed. Second, we report small-angle X-ray scattering data for two S-type DNase pyocins, S2 and AP41, into which are fitted recently determined X-ray structures for isolated domains. We find that DNase pyocins and colicins are both highly elongated molecules, even though the order of their constituent domains differs. We discuss the implications of these architectural similarities and differences in the context of the translocation mechanism of protein antibiotics through the cell envelope of Gram-negative bacteria.

ORCID iDs

Klein, Alexander, Wojdyla, Justyna Aleksandra, Joshi, Amar, Josts, Inokentijs, McCaughey, Laura C., Housden, Nicholas G., Kaminska, Renata, Byron, Olwyn, Walker, Daniel ORCID logoORCID: https://orcid.org/0000-0002-4206-2942 and Kleanthous, Colin;
CORE (COnnecting REpositories)