A photoaffinity displacement assay and probes to study the cyclin‐dependent kinase family

Grant, Emma K. and Fallon, David J. and Eberl, H. Christian and Fantom, Ken G. M. and Zappacosta, Francesca and Messenger, Cassie and Tomkinson, Nicholas C. O. and Bush, Jacob T. (2019) A photoaffinity displacement assay and probes to study the cyclin‐dependent kinase family. Angewandte Chemie International Edition, 58 (48). pp. 17322-17327. ISSN 1433-7851

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    Abstract

    The CDK family plays a crucial role in the control of the cell cycle. Dysregulation and mutation of the CDKs has been implicated in cancer and the CDKs have been investigated extensively as potential therapeutic targets. Selective inhibition of specific isoforms of the CDKs is crucial to achieve therapeutic effect while minimising toxicity. We present a group of photoaffinity probes designed to bind to the family of CDKs. The site of crosslinking of the optimised probe, as well as its ability to enrich members of the CDK family from cell lysates, was investigated. In a proof of concept study, we subsequently developed a photoaffinity probe‐based competition assay to profile CDK inhibitors. We anticipate that this approach will be widely applicable to the study of small molecule binding to protein families of interest.