A novel microfluidic-based approach to formulate size-tuneable large unilamellar cationic liposomes : formulation, cellular uptake and biodistribution investigations
Lou, Gustavo and Anderluzzi, Giulia and Woods, Stuart and Roberts, Craig W. and Perrie, Yvonne (2019) A novel microfluidic-based approach to formulate size-tuneable large unilamellar cationic liposomes : formulation, cellular uptake and biodistribution investigations. European Journal of Pharmaceutics and Biopharmaceutics, 143. pp. 51-60. ISSN 0939-6411 (https://doi.org/10.1016/j.ejpb.2019.08.013)
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Abstract
Extensive research has been undertaken to investigate the effect of liposome size in vitro and in vivo. However, it is often difficult to generate liposomes in different size ranges that offer similar low polydispersity and lamellarity. Conventional methods used in the preparation of liposomes, such as lipid film hydration or reverse phase evaporation, generally give rise to liposomal suspensions displaying broad, multimodal size distribution combined with uncontrolled degree of lamellarity. In contrast, microfluidics allows highly homogeneous liposome dispersions to be produced and adjustment of microfluidic operating parameters (flow rate ratio (FRR) and total flow rate (TFR)) can offer size-tuning of liposomes (up to 300 nm, depending on the formulation). Herein, we demonstrate a novel method which allows the production of highly monodisperse, cationic liposomes over a wide particle size range (up to 750 nm in size). This is achieved through controlling the concentration of the aqueous buffer during production. Using this method, liposomes composed of 1,2-dioleoyl-sn-3-phosphoethanolamine (DOPE) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or dimethyldioctadecylammonium (DDA) – DOPE:DOTAP and DOPE:DDA liposomes – of up to 750 nm were prepared and investigated. Investigating these formulations in vitro demonstrates that cellular uptake of small (40 nm) and large (>500 nm) liposomes in bone marrow-derived macrophages (BMDM) is similar terms of percentage of liposome+ cells and mean fluorescence intensity (MFI). However, significant differences are observed in BMDM uptake when represented in terms of number of liposomes, liposome surface area or liposome internal volume. In vivo biodistribution studies in mice show that by creating small (<50 nm) liposomes we can modify the clearance rates of these liposomes from the injection site and increase accumulation to the draining lymphatics.
ORCID iDs
Lou, Gustavo, Anderluzzi, Giulia, Woods, Stuart ORCID: https://orcid.org/0000-0002-3798-2074, Roberts, Craig W. ORCID: https://orcid.org/0000-0002-0653-835X and Perrie, Yvonne;-
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Item type: Article ID code: 69585 Dates: DateEvent31 October 2019Published22 August 2019Published Online21 August 2019AcceptedSubjects: Medicine > Therapeutics. Pharmacology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 02 Sep 2019 15:14 Last modified: 12 Nov 2024 04:18 URI: https://strathprints.strath.ac.uk/id/eprint/69585