Methodological considerations for large-scale breath analysis studies : lessons from the U-BIOPRED severe asthma project
Ahmed, Waqar M and Brinkman, Paul and Weda, Hans and Knobel, Hugo H and Xu, Yun and Nijsen, Tamara M and Goodacre, Roy and Rattray, Nicholas and Vink, Teunis J and Santonico, Marco and Pennazza, Giorgio and Montuschi, Paulo and Sterk, Peter J and Fowler, Stephen J (2018) Methodological considerations for large-scale breath analysis studies : lessons from the U-BIOPRED severe asthma project. Journal of Breath Research, 13 (1). ISSN 1752-7163
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Abstract
Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.
Creators(s): |
Ahmed, Waqar M, Brinkman, Paul, Weda, Hans, Knobel, Hugo H, Xu, Yun, Nijsen, Tamara M, Goodacre, Roy, Rattray, Nicholas ![]() | Item type: | Article |
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ID code: | 65966 |
Keywords: | metabolomics, quality assurance, breath analysis, volatile organic compounds, asthma, Pharmacy and materia medica, Pulmonary and Respiratory Medicine |
Subjects: | Medicine > Pharmacy and materia medica |
Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
Depositing user: | Pure Administrator |
Date deposited: | 02 Nov 2018 14:05 |
Last modified: | 21 Jan 2021 10:30 |
URI: | https://strathprints.strath.ac.uk/id/eprint/65966 |
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