Time-lapse measurement of single-cell response to nanomaterial : a microfluidic approach

Cunha-Matos, C. A. and Millington, O. M. and Wark, A. W. and Zagnoni, M.; (2016) Time-lapse measurement of single-cell response to nanomaterial : a microfluidic approach. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Chemical and Biological Microsystems Society, IRL, pp. 377-378. ISBN 9781510834163

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    Abstract

    This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution.

    ORCID iDs

    Cunha-Matos, C. A. ORCID logoORCID: https://orcid.org/0000-0001-5528-1725, Millington, O. M. ORCID logoORCID: https://orcid.org/0000-0002-3026-6550, Wark, A. W. ORCID logoORCID: https://orcid.org/0000-0001-8736-7566 and Zagnoni, M. ORCID logoORCID: https://orcid.org/0000-0003-3198-9491;
    CORE (COnnecting REpositories)