Ferric plasmonic nanoparticles, aptamers, and magneto-fluidic chips : toward the development of diagnostic surface-enhanced Raman spectroscopy assays

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Marks, Haley and Huang, Po-Jung and Mabbott, Samuel and Graham, Duncan and Kameoka, Jun and Coté, Gerard (2016) Ferric plasmonic nanoparticles, aptamers, and magneto-fluidic chips : toward the development of diagnostic surface-enhanced Raman spectroscopy assays. Journal of Biomedical Optics, 21 (12). 127005. ISSN 1083-3668 (https://doi.org/10.1117/1.JBO.21.12.127005)

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Abstract

Conjugation of aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound nanoclusters which cause a measurable change in the colloid’s optical properties. In this paper the optimization of a surface enhanced Raman spectroscopy (SERS) competitive binding assay is presented utilizing plasmonic ‘target’ and magnetic ‘probe’ nanoparticles for the detection of the toxin bisphenol-A (BPA). These assay nanoclusters were housed inside three types of optofluidic chips patterned with magnetically-activated nickel pads, in either a straight or array pattern. Both Fe2O3 and Fe2CoO4 were compared as potential magnetic cores for the silver coated probe nanoparticles. We found that the Ag@ Fe2O3 particles were, on average, more uniform in size and more stable than Ag@ Fe2CoO4, whereas the addition of cobalt significantly improved the collection time of particles. Using Raman mapping of the assay housed within the magneto-fluidic chips, it was determined that a 1 x 5 array of 50 µm square nickel pads provided the most uniform SERS enhancement of the assay (coefficient of variation ~25%) within the magneto-fluidic chip. Additionally, the packaged assay demonstrated the desired response to BPA, verifying the technology’s potential to translate magnetic nanoparticle assays into a user-free optical analysis platform.

ORCID iDs

Marks, Haley, Huang, Po-Jung, Mabbott, Samuel ORCID logoORCID: https://orcid.org/0000-0003-4926-5467, Graham, Duncan ORCID logoORCID: https://orcid.org/0000-0002-6079-2105, Kameoka, Jun and Coté, Gerard;
  • Item type:Article
    ID code:58744
    Dates:
    Date
    Event
    20 December 2016
    Published
    17 November 2016
    Accepted
    Notes:Marks, H., Huang, P-J., Mabbott, S., Graham, D., Kameoka, J., & Coté, G. (2016). Ferric plasmonic nanoparticles, aptamers, and magneto-fluidic chips: towards the development of diagnostic SERS assays. Journal of Biomedical Optics, 1-32. Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
    Subjects:Science > Physics > Optics. Light
    Science > Physics > Solid state physics. Nanoscience
    Department:Faculty of Science > Pure and Applied Chemistry
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:18 Nov 2016 12:34
    Last modified:22 Nov 2024 01:11
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/58744
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