Silver colloids as plasmonic substrates for direct label-free surface-enhanced Raman scattering analysis of DNA
Torres-Nuñez, A. and Faulds, K. and Graham, D. and Alvarez-Puebla, R. A. and Guerrini, L. (2016) Silver colloids as plasmonic substrates for direct label-free surface-enhanced Raman scattering analysis of DNA. Analyst, 141 (17). pp. 5170-5180. ISSN 0003-2654 (https://doi.org/10.1039/c6an00911e)
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Abstract
Ultrasensitive direct SERS analysis offers a powerful analytical tool for the structural characterization and classification of nucleic acids. However, acquisition of reliable spectral fingerprints of such complex biomolecules poses important challenges. In recent years, many efforts have been devoted to overcome these limitations, mainly implementing silver colloids as plasmonic substrates. However, a reliable cross-comparison of results reported in the recent literature is extremely hard to achieve, mostly due to the broad set of different surface properties of the plasmonic nanoparticles. Herein, we perform a thorough investigation of the role played by the metal/liquid interface composition of silver colloids in the direct label-free SERS analysis of DNA. Target molecules of increasing complexity, from short homopolymeric strands to long genomic duplexes, were used as probes. We demonstrate how apparently subtle changes in the colloidal surface chemistry can dramatically modify the affinity and the final SERS spectral profile of DNA. This has significant implications for the future design of new analytical strategies for the detection of DNA using SERS without labels.
ORCID iDs
Torres-Nuñez, A., Faulds, K. ORCID: https://orcid.org/0000-0002-5567-7399, Graham, D. ORCID: https://orcid.org/0000-0002-6079-2105, Alvarez-Puebla, R. A. and Guerrini, L.;-
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Item type: Article ID code: 58504 Dates: DateEvent7 September 2016Published12 May 2016Published Online12 May 2016AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry
Technology and Innovation Centre > BionanotechnologyDepositing user: Pure Administrator Date deposited: 09 Nov 2016 11:51 Last modified: 11 Nov 2024 11:31 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/58504