Aptamer conjugated silver nanoparticles for the detection of interleukin 6

Locke, Andrea K. and Norwood, Nicole and Marks, Haley L. and Schechinger, Monika and Jackson, George W. and Graham, Duncan and Coté, Gerard L. (2016) Aptamer conjugated silver nanoparticles for the detection of interleukin 6. In: Plasmonics in Biology and Medicine XIII. Proceedings of SPIE, 9724 . SPIE. ISBN 9781628419580

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    Abstract

    The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ∼42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.

    Author(s):Locke, Andrea K., Norwood, Nicole, Marks, Haley L., Schechinger, Monika, Jackson, George W., Graham, Duncan ORCID logoORCID: https://orcid.org/0000-0002-6079-2105 and Coté, Gerard L.
    Item type:Book Section
    ID code:57995
    Notes:Locke, A. K., Norwood, N., Marks, H. L., Schechinger, M., Jackson, G. W., Graham, D., & Coté, G. L., "Aptamer conjugated silver nanoparticles for the detection of interleukin 6," Plasmonics in Biology and Medicine XIII, T. Vo-Dinh, J. R. Lakowicz, H-P. A. Ho, & K. Ray (Eds.), Proceedings of SPIE; Vol. 9724, 972412 2016. Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2213896
    Keywords:aptamer, binding assay, interleukin 6, plasmonic nanoparticles, protein detection, surface enhance Raman spectroscopy (SERS), biosensors, absorbance, cancer, dynamic light scattering, nano silver particles, optical properties, Bioengineering, Optics. Light, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Biomaterials, Radiology Nuclear Medicine and imaging
    Subjects:Technology > Engineering (General). Civil engineering (General) > Bioengineering
    Science > Physics > Optics. Light
    Department:University of Strathclyde > University of Strathclyde
    Depositing user: Pure Administrator
    Date deposited:30 Sep 2016 10:39
    Last modified:05 Sep 2019 02:30
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/57995
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