Comparison of Fe2O3 and Fe2CoO4 core-shell plasmonic nanoparticles for aptamer mediated SERS assays

Marks, Haley and Mabbott, Samuel and Huang, Po-Jung and Jackson, George W. and Kameoka, Jun and Graham, Duncan and Coté, Gerard L.; Parak, Wolfgang J. and Osinski, Marek and Liang, Xing-Jie, eds. (2016) Comparison of Fe2O3 and Fe2CoO4 core-shell plasmonic nanoparticles for aptamer mediated SERS assays. In: Colloidal Nanoparticles for Biomedical Applications XI. Proceedings of SPIE, 9722 . SPIE, USA. ISBN 9781628419566 (https://doi.org/10.1117/12.2213735)

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Abstract

Conjugation of oligonucleotides or aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound bundles of nanoparticles which cause a measurable change in the colloid's optical properties. Here, we present further optimization of a "SERS off" competitive binding assay utilizing plasmonic and magnetic nanoparticles for the detection of the toxin bisphenol A (BPA). The assay involves 1) a 'target' silver nanoparticle functionalized with a Raman reporter dye and PEGylated BPA-binding DNA aptamers, and 2) a version of the toxin BPA, bisphenol A diglycidyl ether (BADGE), PEGylated and immobilized onto a silver coated magnetic 'probe' nanoparticle. When mixed, these target and probe nanoparticles cluster into magnetic dimers and trimers and an enhancement in their SERS spectra is observed. Upon introduction of free BPA in its native form, target AgNPs are competitively freed; reversing the nanoparticle assembly and causing the SERS signal to "turn-off" and decrease in response to the competitive binding event. The assay particles were housed inside two types of optofluidic chips containing magnetically active nickel pads, in either a straight or spotted pattern, and both Fe2O3 and Fe2CoO4 were compared as magnetic cores for the silver coated probe nanoparticle. We found that the Ag@ Fe2O3 particles were, on average, more uniform in size and more stable than Ag@ Fe2CoO4, while the addition of cobalt significantly improved the collection time of particles within the magnetic chips. Using 3D Raman mapping, we found that the straight channel design with the Ag@ Fe2O3 particles provided the most uniform nanoparticle organization, while the spotted channel design with Ag@ Fe2CoO4 demonstrated a larger SERS enhancement, and thus a lower limit of detection.

  • Item type:Book Section
    ID code:57993
    Dates:
    Date
    Event
    7 March 2016
    Published
    12 October 2015
    Accepted
    Notes:Marks, Haley; Mabbott, Samuel; Huang, Po-Jung; Jackson, George W.; Kameoka, Jun; Graham, Duncan; Coté, Gerard L., "Comparison of Fe2O3 and Fe2CoO4 core-shell plasmonic nanoparticles for aptamer mediated SERS assays," Colloidal Nanoparticles for Biomedical Applications XI. ed. / Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Proceedings of SPIE; Vol. 9722, 97220N (2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2213735
    Subjects:Science > Chemistry
    Technology > Engineering (General). Civil engineering (General)
    Department:University of Strathclyde > University of Strathclyde
    Depositing user: Pure Administrator
    Date deposited:30 Sep 2016 09:39
    Last modified:08 Apr 2024 13:33
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/57993
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