A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging
Trägårdh, J. and Schniete, J. and Parsons, M. and McConnell, G. (2016) A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging. APL Photonics, 1 (9). 091303. ISSN 2378-0967
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Abstract
We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.
Creators(s): |
Trägårdh, J. ![]() ![]() | Item type: | Article |
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ID code: | 57717 |
Keywords: | Raman, lasers, microscopy, live cell, multi-photon, harmonic generation, Optics. Light, Atomic and Molecular Physics, and Optics |
Subjects: | Science > Physics > Optics. Light |
Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
Depositing user: | Pure Administrator |
Date deposited: | 07 Sep 2016 15:34 |
Last modified: | 23 Feb 2021 02:22 |
Related URLs: | |
URI: | https://strathprints.strath.ac.uk/id/eprint/57717 |
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