A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

Trägårdh, J. and Schniete, J. and Parsons, M. and McConnell, G. (2016) A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging. APL Photonics, 1 (9). 091303. ISSN 2378-0967

[img]
Preview
Text (Tragardh-etal-APLP2016-a-femtosecond-raman-generator)
Tragardh_etal_APLP2016_a_femtosecond_raman_generator.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (3MB)| Preview

    Abstract

    We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.