Picture of mobile phone running fintech app

Fintech: Open Access research exploring new frontiers in financial technology

Strathprints makes available Open Access scholarly outputs by the Department of Accounting & Finance at Strathclyde. Particular research specialisms include financial risk management and investment strategies.

The Department also hosts the Centre for Financial Regulation and Innovation (CeFRI), demonstrating research expertise in fintech and capital markets. It also aims to provide a strategic link between academia, policy-makers, regulators and other financial industry participants.

Explore all Strathclyde Open Access research...

Two-color, two-photon imaging at long excitation wavelengths using a diamond Raman laser

Tragardh, Johanna and Murtagh, Michelle and Robb, Gillian and Parsons, Maddy and Lin, Jipeng and Spence, David J. and McConnell, Gail (2016) Two-color, two-photon imaging at long excitation wavelengths using a diamond Raman laser. Microscopy and Microanalysis. ISSN 1431-9276

Text (Tragardh-etal-MM-2016-two-color-two-photon-imaging-at-long-excitation-wavelengths)
Accepted Author Manuscript
License: Unspecified

Download (329kB) | Preview


We demonstrate that the second-Stokes output from a diamond Raman laser, pumped by a femtosecond Ti:Sapphire laser, can be used to efficiently excite red-emitting dyes by two-photon excitation at 1080 nm and beyond. We image red fluorescent protein (RFP) expressing HeLa cells, as well as dyes such as Texas Red and Mitotracker Red. We demonstrate the potential for simultaneous two-color, two-photon imaging with this laser by using the residual pump beam for excitation of a green-emitting dye. We demonstrate this for the combination of AlexaFluor 488 and AlexaFluor 568. Because the Raman laser extends the wavelength range of the Ti:Sapphire laser, resulting in a laser system tunable 680 nm-1200 nm it can be used for two-photon excitation of a large variety and combination of dyes.