Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS
Tusiimire, Jonans and Wallace, Jennifer and Woods, Nicola and Dufton, Mark J. and Parkinson, John A. and Abbott, Grainne and Clements, Carol J. and Young, Louise and Park, Jin Kyu and Jeon, Jong Woon and Ferro, Valerie A. and Watson, David G. (2016) Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS. Vaccines, 4 (2). 11. ISSN 2076-393X (https://doi.org/10.3390/vaccines4020011)
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Abstract
The venom of Apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. Four fractions from whole bee venom (BV) were separated using medium pressure liquid chromatography. Their ability to induce the production of cytokines TNFα, IL-1β and IL-6 in phorbol-12-myristate-13-acetate (PMA)-treated U937 cells was assessed. The levels of the three cytokines produced by stimulation with the four fractions and crude BV without LPS were not significantly different from negative control values. However, co-stimulation of the cells with LPS and Fraction 4 (F-4) induced a 1.6-fold increase in TNF-α level (p < 0.05) compared to LPS alone. Likewise, LPS-induced IL-1β production was significantly synergised in the presence of F-1 (nine-fold), F-2 (six-fold), F-3 (four-fold) and F-4 (two-fold) fractions, but was only slightly enhanced with crude BV (1.5-fold) relative to LPS. Furthermore, the LPS-stimulated production of IL-6 was not significantly increased in cells co-treated with F-2 and F-3, but the organic fraction (F-4) showed an inhibitory effect (p < 0.05) on IL-6 production. The latter was elucidated by NMR spectroscopy and found to contain(Z)-9-eicosen-1-ol. The effects observed with the purified BV fractions were more marked than those obtained with the crude sample.
ORCID iDs
Tusiimire, Jonans ORCID: https://orcid.org/0000-0002-5831-1499, Wallace, Jennifer ORCID: https://orcid.org/0000-0003-4165-3393, Woods, Nicola, Dufton, Mark J. ORCID: https://orcid.org/0000-0001-8176-2194, Parkinson, John A. ORCID: https://orcid.org/0000-0003-4270-6135, Abbott, Grainne, Clements, Carol J., Young, Louise, Park, Jin Kyu, Jeon, Jong Woon, Ferro, Valerie A. ORCID: https://orcid.org/0000-0003-1967-3603 and Watson, David G. ORCID: https://orcid.org/0000-0003-1094-7604;-
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Item type: Article ID code: 56334 Dates: DateEvent19 April 2016Published13 April 2016AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Pure and Applied ChemistryDepositing user: Pure Administrator Date deposited: 10 May 2016 10:30 Last modified: 06 Oct 2024 00:28 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/56334