Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced raman spectroscopy

Simpson, Jonathan and Craig, Derek and Faulds, Karen and Graham, Duncan (2016) Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced raman spectroscopy. Nanoscale Horizons (1). pp. 60-63.

[img]
Preview
Text (Simpson-etal-NH-2015-Mixed-monolayer-glyconanoparticles-for-the-detection-of-cholera-toxin-by-surface-enhanced)
Simpson_etal_NH_2015_Mixed_monolayer_glyconanoparticles_for_the_detection_of_cholera_toxin_by_surface_enhanced.pdf
Final Published Version
License: Creative Commons Attribution 3.0 logo

Download (1MB)| Preview

    Abstract

    The same interactions that a pathogen uses for establishment in a host can be exploited in its detection. The carbohydrates comprising the intestinal cell surface GM1-ganglioside, are targeted by vibrio cholerae via the lectin, cholera toxin, to initiate infection. We report on the preparation of mixed-monolayer, carbohydrate-coated silver nanoparticles (glyconanoparticles) for the sensitive (56 ng/mL), low volume detection of cholera toxin B-subunit (CTB) in synthetic freshwater samples and in 5 minutes by surface enhanced Raman spectroscopy (SERS). The detection limit falls within the recommended detection range and matches WHO approved test limits. PEGylated galactose and sialic acid are added in a specific ratio to coat the particles in GM1-ganglioside mimics for interaction with CTB and display a synergic effect greater than either glycan alone. This demonstrates the first use of a mixed-monolayer glyconanoparticle which mimics the GM1 ligand, allowing selective interaction with CTB.