Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polypropylenimine polyplex
Aldawsari, Hibah Mobarak and Edrada-Ebel, Ruangelie and Blatchford, David and Tate, Rothwelle and Tetley, Laurence and Dufès, Christine (2011) Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polypropylenimine polyplex. Biomaterials, 32 (25). pp. 5889-5899. ISSN 1878-5905 (https://doi.org/10.1016/j.biomaterials.2011.04.079)
Full text not available in this repository.Request a copyAbstract
The possibility of using non-viral gene delivery systems for the treatment of cancer is currently limited by their lower transfection efficacy compared to viral systems. On the basis that amino acids such as arginine, lysine and leucine were involved in enhancing DNA transportation into cells, we hypothesized that the grafting of these amino acids to the highly promising generation 3 diaminobutyric polypropylenimine (DAB) dendrimer would improve its transfection efficacy in cancer cells. In this work we demonstrated that the conjugation of arginine, lysine and leucine to the dendrimer led to an enhanced anti-proliferative activity of the polyplexes, by up to 47-fold for DAB-Lys in T98G cancer cells compared to the unmodified polyplex in vitro. In vivo, the intravenous administration of amino acid-bearing DAB polyplexes resulted in a significantly improved tumor gene expression, with the highest gene expression level observed after treatment with DAB-Lys polyplex. Arginine, lysine and leucine-bearing generation 3 polypropylenimine polymers are therefore highly promising gene delivery systems for gene transfection in tumors.
ORCID iDs
Aldawsari, Hibah Mobarak, Edrada-Ebel, Ruangelie, Blatchford, David, Tate, Rothwelle, Tetley, Laurence and Dufès, Christine ORCID: https://orcid.org/0000-0002-7963-6364;-
-
Item type: Article ID code: 31793 Dates: DateEventSeptember 2011Published18 May 2011Published OnlineSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Technology and Innovation Centre > BionanotechnologyDepositing user: Pure Administrator Date deposited: 16 Jun 2011 10:48 Last modified: 11 Nov 2024 09:47 URI: https://strathprints.strath.ac.uk/id/eprint/31793