Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

McKenzie, F. and Steven, V. and Ingram, A. and Graham, D. (2009) Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis. Chemical Communications (London) (20). pp. 2872-2874. ISSN 0009-241X

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Abstract

A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.