A modelling study of a non-concerted hydrolytic cycloaddition reaction by the catalytic antibody H11

Clark, R.L. and Johnston, B.F. and Suckling, C.J. and Mackay, S.P. (2006) A modelling study of a non-concerted hydrolytic cycloaddition reaction by the catalytic antibody H11. Bioorganic and Medicinal Chemistry Letters, 14. pp. 2674-2683. ISSN 0960-894X

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Abstract

H11 is the first antibody reported to have dual activity as a non-concerted, Diels-Alderase and hydrolytic catalyst. It was previously shown to catalyse the cycloaddition of acetoxybutadiene 1a to N-alkyl maleimides 2 to afford hydroxy-substituted bicyclic adducts 3 with a 30% ee of a major isomer. To better understand this mechanism and the partial stereospecificity, a homology model of H11 was constructed and used in docking studies to evaluate potential antibody-ligand complexes. The model suggested the hydrolytic nature of H11 was due to Glu 95H acting as a catalytic base, and evaluation of the shape complementarity of the proposed antibody-ligand complexes confirmed at a semi-quantitative level the observation that the major enantiomer is produced in a 30% ee.

Author(s):Clark, R.L., Johnston, B.F. ORCID logoORCID: https://orcid.org/0000-0001-9785-6822, Suckling, C.J. and Mackay, S.P. ORCID logoORCID: https://orcid.org/0000-0001-8000-6557
Item type:Article
ID code:10624
Keywords:modelling, cycloaddition, hydrolysis, antibody, Diels-Alderase, Pharmacy and materia medica, Biochemistry, Organic Chemistry, Drug Discovery, Pharmaceutical Science, Molecular Medicine, Molecular Biology, Clinical Biochemistry
Subjects:Medicine > Pharmacy and materia medica
Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Pure and Applied Chemistry
Depositing user: Strathprints Administrator
Date deposited:01 Oct 2010 18:32
Last modified:13 Feb 2020 07:01
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URI:https://strathprints.strath.ac.uk/id/eprint/10624
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