Exploring bioinformatics-driven approaches for enhanced diagnosis of Chlamydia trachomatis infections : analysis of target proteins

Oladipo, Elijah Kolawole and Oluwasegun, Jerry Ayobami and Kibathi, Mercy Hokah and Nwonu, Ezinne Janefrances and Omerigwe, Simon Achi and Olatunde, Simeon Kayowa and Akinyemi, Daniel Funsho and Aminu, Ruth Foluke and Hussaini, Muhammad Awwal and Ockiya, Michael Asebake and Liadi, Ahmad Mansur and Osikoya, Opeyemi Olayinka and Kolawole, Eniola Olaitan and Obideyi, Oluwaseun Adeola and Bello, Taye Olusola and Tadasare, Niraj Nivas (2024) Exploring bioinformatics-driven approaches for enhanced diagnosis of Chlamydia trachomatis infections : analysis of target proteins. EMJ Innovations. ISSN 2513-8634 (https://doi.org/10.33590/emjinnov/htqi2830)

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Abstract

Chlamydia trachomatis, a major cause of sexually transmitted infection, poses a range of symptoms including genital discharge, pain during urination, and abdominal pains in women, and can lead to serious health complications such as pelvic inflammatory diseases, infertility, and ectopic pregnancy if left untreated. The need for rapid and accurate detection is imperative so prompt treatment and control of the disease can be achieved. This study conducted an immunoinformatic analysis of proteins of Chlamydia trachomatis (incA, hctA, ompA, omcB, rpoB, and HSP60) for the development of a lateral flow assay-based diagnostic test. Detailed in silico evaluation of selected proteins from publicly available genomic databases was conducted to evaluate their suitability as targets for lateral flow assay-based detection. The series of tests included antigenicity, toxicity, solubility, physicochemical characteristics and molecular docking of the derived constructs, and protein sequence. Chimeric construct was derived from the prediction of linear B cell epitopes, helper T cell major histocompatibility complex II binding epitopes, and IL4 and IL10 inducers using bioinformatic tools at standard thresholds. With a Ramachandra’s score of 95.4% and Z-score of -5.1, results indicate that the construct efficacy is high in potential to provide extreme specificity and sensitivity for the detection of Chlamydia trachomatis in clinical samples as compared to traditional culture-based methods using nucleic acid amplification, hereby providing a quicker and more accurate diagnostic tool for Chlamydia trachomatis infection. Findings offer valuable data for the development of a rapid and reliable diagnostic point-of-care test kit for Chlamydia trachomatis that allows for drastic reduction in clinical wait time and treatment.

ORCID iDs

Oladipo, Elijah Kolawole, Oluwasegun, Jerry Ayobami, Kibathi, Mercy Hokah, Nwonu, Ezinne Janefrances, Omerigwe, Simon Achi ORCID logoORCID: https://orcid.org/0009-0000-6419-7672, Olatunde, Simeon Kayowa, Akinyemi, Daniel Funsho, Aminu, Ruth Foluke, Hussaini, Muhammad Awwal, Ockiya, Michael Asebake, Liadi, Ahmad Mansur, Osikoya, Opeyemi Olayinka, Kolawole, Eniola Olaitan, Obideyi, Oluwaseun Adeola, Bello, Taye Olusola and Tadasare, Niraj Nivas;
CORE (COnnecting REpositories)