Vertical and horizontal transmission of cell fusing agent virus in Aedes aegypti

Logan, Rhiannon A.E. and Quek, Shannon and Muthoni, Joseph N. and von Eicken, Anneliese and Brettell, Laura E. and Anderson, Enyia R. and Villena, Marcus E.N. and Hegde, Shivanand and Patterson, Grace T. and Heinz, Eva and Hughes, Grant L. and Patterson, Edward I. (2022) Vertical and horizontal transmission of cell fusing agent virus in Aedes aegypti. Applied and Environmental Microbiology, 88 (18). e01062-22. ISSN 0099-2240 (https://doi.org/10.1128/aem.01062-22)

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Abstract

Cell fusing agent virus (CFAV) is an insect-specific flavivirus (ISF) found in Aedes aegypti mosquitoes. ISFs have demonstrated the ability to modulate the infection or transmission of arboviruses such as dengue, West Nile, and Zika viruses. It is thought that vertical transmission is the main route for ISF maintenance in nature. This has been observed with CFAV, but there is evidence of horizontal and venereal transmission in other ISFs. Understanding the route of transmission can inform strategies to spread ISFs to vector populations as a method of controlling pathogenic arboviruses. We crossed individually reared male and female mosquitoes from both a naturally occurring CFAV-positive Ae. aegypti colony and its negative counterpart to provide information on maternal, paternal, and horizontal transmission. RT-PCR was used to detect CFAV in individual female pupal exuviae and was 89% sensitive, but only 42% in male pupal exuviae. This is a possible way to screen individuals for infection without destroying the adults. Female-to-male horizontal transmission was not observed during this study. However, there was a 31% transmission rate from mating pairs of CFAV-positive males to negative female mosquitoes. Maternal vertical transmission was observed with a filial infection rate of 93%. The rate of paternal transmission was 85% when the female remained negative, 61% when the female acquired CFAV horizontally, and 76% overall. Maternal and paternal transmission of CFAV could allow the introduction of this virus into wild Ae. aegypti populations through male or female mosquito releases, and thus provides a potential strategy for ISF-derived arbovirus control. IMPORTANCE Insect-specific flaviviruses (ISFs), are a group of nonpathogenic flaviviruses that only infect insects. ISFs can have a high prevalence in mosquito populations, but their transmission routes are not well understood. The results of this study confirm maternal transmission of cell fusing agent virus (CFAV) and demonstrate that paternal transmission is also highly efficient. Horizontal transmission of CFAV was also observed, aided by evaluation of the pupal infection status before mating with an infected individual. This technique of detecting infection in discarded pupae exuviae has not been evaluated previously and will be a useful tool for others in the field of studying viral transmission in mosquitoes. Identifying these routes of transmission provides information about how CFAV could be maintained in wild populations of mosquitoes and can aid future studies focusing on interactions of CFAV with their hosts and other viruses that infect mosquitoes.

ORCID iDs

Logan, Rhiannon A.E., Quek, Shannon, Muthoni, Joseph N., von Eicken, Anneliese, Brettell, Laura E., Anderson, Enyia R., Villena, Marcus E.N., Hegde, Shivanand, Patterson, Grace T., Heinz, Eva ORCID logoORCID: https://orcid.org/0000-0003-4413-3756, Hughes, Grant L. and Patterson, Edward I.;
  • Item type:Article
    ID code:90648
    Dates:
    Date
    Event
    22 September 2022
    Published
    29 August 2022
    Published Online
    5 August 2022
    Accepted
    Notes:Copyright © 2022 American Society for Microbiology. All Rights Reserved.
    Subjects:UNSPECIFIED
    Department:UNSPECIFIED
    Depositing user: Pure Administrator
    Date deposited:23 Sep 2024 12:48
    Last modified:11 Nov 2024 14:27
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/90648
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