Magnetic graphene oxide nanocomposites for selective miRNA separation and recovery

Uten, Supapitch and Boonbanjong, Poramin and Prueksathaporn, Yosaphon and Treerattrakoon, Kiatnida and Sathirapongsasuti, Nuankanya and Chanlek, Narong and Pinitsoontorn, Supree and Luksirikul, Patraporn and Japrung, Deanpen (2024) Magnetic graphene oxide nanocomposites for selective miRNA separation and recovery. ACS Omega, 9 (2). pp. 2263-2271. ISSN 2470-1343 (https://doi.org/10.1021/acsomega.3c05919)

[thumbnail of Uten-etal-ACS-Omega-2024-Magnetic-graphene-oxide-nanocomposites-for-selective-miRNA-separation]
Preview
 Text. Filename: Uten-etal-ACS-Omega-2024-Magnetic-graphene-oxide-nanocomposites-for-selective-miRNA-separation.pdf
Final Published Version
License: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 logo
Download (4MB)| Preview

Abstract

In this study, we developed magnetic graphene oxide composites by chemically attaching Fe3O4 nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe3O4@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.

  • Item type:Article
    ID code:89110
    Dates:
    Date
    Event
    16 January 2024
    Published
    2 January 2024
    Published Online
    18 December 2023
    Accepted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:03 May 2024 13:35
    Last modified:11 Nov 2024 14:18
    URI:https://strathprints.strath.ac.uk/id/eprint/89110
CORE (COnnecting REpositories)