Surface functionalisation of self-assembled quantum dot microlasers with a DNA aptamer

Charlton, Bethan K. and Downie, Dillon H. and Noman, Isaac and Urbano Alves, Pedro and Eling, Charlotte J. and Laurand, Nicolas (2023) Surface functionalisation of self-assembled quantum dot microlasers with a DNA aptamer. International Journal of Molecular Sciences, 24 (19). 14416. ISSN 1422-0067 (https://doi.org/10.3390/ijms241914416)

[thumbnail of Charltonet-al-IJMS-2023-Surface-functionalisation-of-self-assembled-quantum-dot-microlasers]
Preview
 Text. Filename: Charltonet_al_IJMS_2023_Surface_functionalisation_of_self_assembled_quantum_dot_microlasers.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo
Download (4MB)| Preview

Abstract

The surface functionalisation of self-assembled colloidal quantum dot supraparticle lasers with a thrombin binding aptamer (TBA-15) has been demonstrated. The self-assembly of CdSSe/ZnS alloyed core/shell microsphere-shape CQD supraparticles emitting at 630 nm was carried out using an oil-in-water emulsion technique, yielding microspheres with an oleic acid surface and an average diameter of 7.3 ± 5.3 µm. Surface modification of the microspheres was achieved through a ligand exchange with mercaptopropionic acid and the subsequent attachment of TBA-15 using EDC/NHS coupling, confirmed by zeta potential and Fourier transform IR spectroscopy. Lasing functionality between 627 nm and 635 nm was retained post-functionalisation, with oleic acid- and TBA-coated microspheres exhibiting laser oscillation with thresholds as low as 4.10 ± 0.37 mJ·cm−2 and 7.23 ± 0.78 mJ·cm−2, respectively.

ORCID iDs

Charlton, Bethan K. ORCID logoORCID: https://orcid.org/0009-0000-8484-3512, Downie, Dillon H. ORCID logoORCID: https://orcid.org/0009-0005-0435-0344, Noman, Isaac, Urbano Alves, Pedro ORCID logoORCID: https://orcid.org/0000-0003-3447-6878, Eling, Charlotte J. ORCID logoORCID: https://orcid.org/0000-0002-3819-2728 and Laurand, Nicolas ORCID logoORCID: https://orcid.org/0000-0003-0486-4300;
CORE (COnnecting REpositories)