Using the Mesolens to observe structural changes in E. coli mature colony biofilms under different nutrient availability

Bottura, Beatrice and Hoskisson, Paul and McConnell, Gail (2021) Using the Mesolens to observe structural changes in E. coli mature colony biofilms under different nutrient availability. In: SUPA Virtual Gathering, 2021-05-25 - 2021-05-27.

[thumbnail of Bottura-etal-SUPA-2021-Using-the-Mesolens-to-observe-structural-changes-in-E-coli-mature-colony-biofilms]
Preview
 Text. Filename: Bottura_etal_SUPA_2021_Using_the_Mesolens_to_observe_structural_changes_in_E_coli_mature_colony_biofilms.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo
Download (337kB)| Preview

Abstract

The morphology of bacterial colonies depends on nutrient availability . We use the Mesolens , which can image a 100 mm 3 volume with subcellular resolution throughout, to investigate the internal structure of colonies grown on agar substrates with different glucose and ammonia concentrations. We observe intra colony channels in both nutrient deprived and nutrient rich colonies, with channel widths dependent on the nutrient availability.

ORCID iDs

Bottura, Beatrice ORCID logoORCID: https://orcid.org/0000-0001-7114-4384, Hoskisson, Paul ORCID logoORCID: https://orcid.org/0000-0003-4332-1640 and McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
CORE (COnnecting REpositories)