Kinetic analysis of bioorthogonal reaction mechanisms using Raman microscopy

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Tipping, William J. and Lee, Martin and Brunton, Valerie G. and Lloyd-Jones, Guy C. and Hulme, Alison N. (2019) Kinetic analysis of bioorthogonal reaction mechanisms using Raman microscopy. Faraday Discussions, 220. pp. 71-85. ISSN 1359-6640 (https://doi.org/10.1039/c9fd00057g)

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Abstract

Raman spectroscopy is well-suited to the study of bioorthogonal reaction processes because it is a non-destructive technique, which employs relatively low energy laser irradiation, and water is only very weakly scattered in the Raman spectrum enabling live cell imaging. In addition, Raman spectroscopy allows species-specific label-free visualisation; chemical contrast may be achieved when imaging a cell in its native environment without fixatives or stains. Combined with the rapid advances in the field of Raman imaging over the last decade, particularly in stimulated Raman spectroscopy (SRS), this technique has the potential to revolutionise our mechanistic understanding of the biochemical and medicinal chemistry applications of bioorthogonal reactions. Current approaches to the kinetic analysis of bioorthogonal reactions (including heat flow calorimetry, UV-vis spectroscopy, fluorescence, IR, NMR and MS) have a number of practical shortcomings for intracellular applications. We highlight the advantages offered by Raman microscopy for reaction analysis in the context of both established and emerging bioorthogonal reactions, including the copper(i) catalysed azide-alkyne cycloaddition (CuAAC) click reaction and Glaser-Hay coupling.

  • Item type:Article
    ID code:84510
    Dates:
    Date
    Event
    2 September 2019
    Published
    18 June 2019
    Accepted
    Notes:Funding Information: The research leading to these results received funding from the BBSRC (Grant Ref: BB/N021614/1), Cancer Research UK (Grant ref: C157/A25140 and C157/A15703), and the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement 340163. We thank Dr Colin Campbell for use of the spontaneous Raman microscope [UK Regenerative Medicine Platform Niche Hub, MR/K026666/1]. Funding Information: The research leading to these results received funding from the BBSRC (Grant Ref: BB/N021614/1), Cancer Research UK (Grant ref: C157/A25140 and C157/A15703), and the European Research Council under the European Union’s Seventh Framework Programme (FP7/2007–2013)/ERC Grant Agreement 340163. We thank Dr Colin Campbell for use of the spontaneous Raman microscope [UK Regenerative Medicine Platform Niche Hub, MR/K026666/1]. Publisher Copyright: © 2019 The Royal Society of Chemistry. Faraday Discuss., 2019,220, 71-85
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:02 Mar 2023 13:26
    Last modified:11 Nov 2024 13:49
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/84510
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