Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation

Muir, Rhona and Forbes, Shareen and Birch, David J S and Vyshemirsky, Vladislav and Rolinski, Olaf J (2023) Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation. Methods and Applications in Fluorescence, 11 (1). 015003. ISSN 2050-6120 (https://doi.org/10.1088/2050-6120/aca507)

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Abstract

We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steadystate and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro. The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.

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