Polyphenol-rich leaf of annona squamosa stimulates insulin release from BRIN-BD11 cells and isolated mouse islets, reduces (CH2O)n digestion and absorption, and improves glucose tolerance and GLP-1 (7-36) levels in high-fat- fed rats

Ansari, Prawej and Hannan, J.M.A. and Seidel, Veronique and Abdel-Wahab, Yaser H.A. (2022) Polyphenol-rich leaf of annona squamosa stimulates insulin release from BRIN-BD11 cells and isolated mouse islets, reduces (CH2O)n digestion and absorption, and improves glucose tolerance and GLP-1 (7-36) levels in high-fat- fed rats. Metabolites, 12 (10). 995. ISSN 2218-1989 (https://doi.org/10.3390/metabo12100995)

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Abstract

Annona squamosa, commonly known as custard apple, is traditionally used for the treat-ment of various diseases including diabetes, cardiovascular disease (CVD), and gastritis. This study was undertaken to investigate the effects of an ethanolic (80% v/v) extract of A. squamosa (EEAS) leaves in vitro on insulin secretion from clonal pancreatic BRIN BD11 β-cells and mouse islets, in-cluding mechanistic studies on the effect of EEAS on membrane potential and intracellular calcium ion concentration. Additional in vitro glucose-lowering actions were assessed. For in vivo studies, high-fat-fed (HFF) obese/normal rats were selected. EEAS increased insulin secretion in vitro in a dose-dependent manner. This effect was linked to β-cell membrane depolarisation and cytoplasmic Ca2+ influx. In the presence of isobutyl methylxanthine (IBMX), tolbutamide, or KCl, the insulin-releasing effect of EEAS was increased, suggesting its effect was also mediated via a KATP-independ-ent pathways. EEAS inhibited insulin glycation, glucose absorption, and DPP-IV enzyme activity in vitro and enhanced glucose uptake and insulin action in 3T3L1 cells. In vivo, gut motility, food intake, glucose tolerance, plasma insulin, and active GLP-1 (7-36) levels were improved, whereas plasma DPP-IV levels were reduced in HFF rats. EEAS attenuated the absorption of sucrose and glucose as well as decreased serum glucose levels after sucrose loading and in situ intestinal perfu-sion in non-diabetic rats. Rutin, proanthocyanidin, and squafosacin G were putatively identified as the anti-hyperglycaemic phytomolecules in EEAS using HPLC followed by LC-MS analysis. This study illustrates the potential of A. squamosa and its phytoconstituents as a source of potential anti-diabetic agents.

ORCID iDs

Ansari, Prawej, Hannan, J.M.A., Seidel, Veronique ORCID logoORCID: https://orcid.org/0000-0003-3880-5261 and Abdel-Wahab, Yaser H.A.;
CORE (COnnecting REpositories)