Engineered myoglobin as a catalyst for atom transfer radical cyclisation
Lubskyy, Andriy and Guo, Chao and Chadwick, Robert J. and Petri-Fink, Alke and Bruns, Nico and Pellizzoni, Michela M. (2022) Engineered myoglobin as a catalyst for atom transfer radical cyclisation. Chemical Communications, 58 (78). pp. 10989-10992. ISSN 1359-7345 (https://doi.org/10.1039/d2cc03227a)
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Abstract
Myoglobin was subjected to site-directed mutagenesis and transformed into a catalyst able to perform atom transfer radical cyclisation reactions, i.e. intramolecular atom transfer radical additions. Replacing the iron-coordinating histidine with serine, or introducing small changes inside or at the entrance of the active site, transformed the completely inactive wild-type myoglobin into an artificial metalloenzyme able to catalyse the 5-exo cyclisation of halogenated unsaturated compounds for the synthesis of γ-lactams. This new-to-nature activity was achieved not only with purified protein but also in crude cell lysate and in whole cells.
ORCID iDs
Lubskyy, Andriy, Guo, Chao, Chadwick, Robert J., Petri-Fink, Alke, Bruns, Nico ORCID: https://orcid.org/0000-0001-6199-9995 and Pellizzoni, Michela M.;-
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Item type: Article ID code: 82491 Dates: DateEvent31 August 2022Published31 August 2022Published Online30 August 2022AcceptedSubjects: Science > Chemistry
Technology > Chemical engineeringDepartment: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 29 Sep 2022 14:43 Last modified: 11 Nov 2024 13:38 URI: https://strathprints.strath.ac.uk/id/eprint/82491