Nanopore extended field-effect transistor for selective single-molecule biosensing

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Ren, Ren and Zhang, Yanjun and Paulose Nadappuram, Binoy and Akpinar, Bernice and Klenerman, David and Ivanov, Aleksandar P. and Edel, Joshua B. and Korchev, Yuri (2017) Nanopore extended field-effect transistor for selective single-molecule biosensing. Nature Communications, 8. 586. ISSN 2041-1723 (https://doi.org/10.1038/s41467-017-00549-w)

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Abstract

There has been a significant drive to deliver nanotechnological solutions to biosensing, yet there remains an unmet need in the development of biosensors that are affordable, integrated, fast, capable of multiplexed detection, and offer high selectivity for trace analyte detection in biological fluids. Herein, some of these challenges are addressed by designing a new class of nanoscale sensors dubbed nanopore extended field-effect transistor (nexFET) that combine the advantages of nanopore single-molecule sensing, field-effect transistors, and recognition chemistry. We report on a polypyrrole functionalized nexFET, with controllable gate voltage that can be used to switch on/off, and slow down single-molecule DNA transport through a nanopore. This strategy enables higher molecular throughput, enhanced signal-to-noise, and even heightened selectivity via functionalization with an embedded receptor. This is shown for selective sensing of an anti-insulin antibody in the presence of its IgG isotype.

ORCID iDs

Ren, Ren, Zhang, Yanjun, Paulose Nadappuram, Binoy ORCID logoORCID: https://orcid.org/0000-0002-1386-8357, Akpinar, Bernice, Klenerman, David, Ivanov, Aleksandar P., Edel, Joshua B. and Korchev, Yuri;
  • Item type:Article
    ID code:81699
    Dates:
    Date
    Event
    19 September 2017
    Published
    7 July 2017
    Accepted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:03 Aug 2022 15:10
    Last modified:14 Dec 2024 01:32
    URI:https://strathprints.strath.ac.uk/id/eprint/81699
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