Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution
Corsetti, Stella and Wijesinghe, Philip and Poulton, Persephone B. and Sakata, Shuzo and Vyas, Khushi and Herrington, C. Simon and Nylk, Jonathan and Gasparoli, Federico and Dholakia, Kishan (2020) Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution. OSA Continuum, 3 (4). pp. 1068-1083. ISSN 2770-0208 (https://doi.org/10.1364/OSAC.391644)
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Abstract
Imaging across length scales and in depth has been an important pursuit of widefield optical imaging. This promises to reveal fine cellular detail within a widefield snapshot of a tissue sample. Current advances often sacrifice resolution through selective sub-sampling to provide a wide field of view in a reasonable time scale. We demonstrate a new avenue for recovering high-resolution images from sub-sampled data in light sheet microscopy using deep-learning super-resolution. We combine this with the use of a widefield Airy beam to achieve high-resolution imaging over extended fields of view and depths. We characterise our method on fluorescent beads as test targets. We then demonstrate improvements in imaging amyloid plaques in a cleared brain from a mouse model of Alzheimer’s disease, and in excised healthy and cancerous colon and breast tissues. This development can be widely applied in all forms of light sheet microscopy to provide a two-fold increase in the dynamic range of the imaged length scale. It has the potential to provide further insight into neuroscience, developmental biology, and histopathology.
ORCID iDs
Corsetti, Stella, Wijesinghe, Philip, Poulton, Persephone B., Sakata, Shuzo ORCID: https://orcid.org/0000-0001-6796-411X, Vyas, Khushi, Herrington, C. Simon, Nylk, Jonathan, Gasparoli, Federico and Dholakia, Kishan;-
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Item type: Article ID code: 75373 Dates: DateEvent14 April 2020Published2 April 2020AcceptedSubjects: Medicine > Pharmacy and materia medica
Science > Physics > Optics. LightDepartment: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 11 Feb 2021 12:00 Last modified: 18 Sep 2024 05:51 URI: https://strathprints.strath.ac.uk/id/eprint/75373